RESEARCH USE ONLY Electrochemical measurement · EFIRM technology
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EZLIFE BIO · ELECTROCHEMICAL RESEARCH

EFIRM96Electrochemical research platform

A different way to read
the molecular world.

EZLife Bio is an upstream technology platform company. EFIRM (Electric Field-Induced Release and Measurement) connects EFIRM96 instruments, EPLATE consumables and software for research and target-specific assay kit development.

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EFIRM96 research instrument, showing its enclosure, touchscreen and EZLife markings
Field-assisted recognition.
Electrochemical measurement.Research use only
Not for diagnostic procedures

01 / THE PLATFORM

One platform.
Three core products.

Use the platform for molecular research or build your own target-specific assay kits, combining recognition chemistry with instrumentation, electrode consumables and software.

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EFIRM96 instrument with its original enclosure and controls

EFIRM96

Instrumentation for parallel electrochemical research measurements.

Explore the instrumentOriginal plate handling in a 2020 EFIRM research workflow

EPLATE

Electrode plate consumables provide the sensing surface for assay-specific recognition.

Explore consumablesEarlier EFIRM plate-layout software workspace alongside the instrument, 2020

Software

Connect instrument operation, plate layout and measurement data.

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02 / THE SCIENCE

Recognition becomes
an electrical signal.

EFIRM integrates a conducting-polymer probe interface, electric-field modulated molecular binding and HRP–TMB electrochemical signal amplification. Selected nucleic acid workflows detect targets without polymerase chain reaction (PCR) amplification.

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Three technologies working together

Build the recognition interfaceIsometric molecular schematic: capture probes incorporated in a conducting-polymer layer on a gold working electrode, cyclic waveform-assisted target hybridization, and a biotinylated detector linked through streptavidin to HRP. TMB redox cycling generates current without copying DNA.Capture probesElectropolymerized PPyGold working electrodePyrrole monomersElectropolymerization waveformPyrrolemonomersCaptureprobesPPy interfaceGold electrode
  • Capture probe
  • Conducting polymer
  • Working electrode
  • Pyrrole → PPy interface
TECHNOLOGY 1 / 3

Build the recognition interface

An applied electrical waveform polymerizes a conducting-polymer layer at the gold working electrode, incorporating capture probes at the sensing interface. Published nucleic acid methods use polypyrrole (PPy).

The electrode, polymer and molecular probes form one functional interface. This schematic illustrates a published nucleic acid assay; surface formulations vary by method.

Published nucleic acid assay schematic · not to scale. Molecular motion and the current trace illustrate the mechanism. Release and sample preparation are assay-specific; washes and controls are omitted from the drawing.

PCR-free target detection

Enzyme-mediated signal generation is distinct from copying DNA. Chemistry, sample preparation and performance depend on the assay and its validated protocol.

03 / THE EVIDENCE

The science moves forward.
Follow the research.

Selected EFIRM and related liquid biopsy publications across research groups. Original studies and reviews are identified separately, with their limits in view.

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2024Original research

Scientific Reports · 14:30792

Direct detection of 4-dimensions of SARS-CoV-2: infection (vRNA), infectivity (antigen), binding antibody, and functional neutralizing antibody in saliva

Investigates four separate electrochemical assays for viral RNA, antigen, binding antibody and neutralizing antibody signals in saliva.

Separate singleplex tests, rather than four targets measured simultaneously in one well. The viral RNA workflow includes RT-LAMP amplification and restriction digestion; antibody assays use distinct preparation and readout conditions. Not a clinical testing authorization.

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AVAILABLE PLATFORM PRODUCTS

Ready for your
next research project.

Existing EFIRM96 instruments, EPLATE consumables and instrument software are available for delivery.

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Larger orders may require contract manufacturing. Lead times depend on order size, configuration and production arrangements and are confirmed during quotation.

Included with the instrument

Instrument purchase includes software for use on that instrument only, training and general technical support.

Develop a method together

For assay-development guidance, we prioritize discussing collaboration; paid support may also be arranged on a project-by-project basis.

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MITOCHONDRIAL DNA · RESEARCH DIRECTION

Measure selected mtDNA targets
without copying the DNA.

Published meat-species research demonstrates direct, PCR-free measurement of selected mitochondrial DNA targets after sample treatment.

A defined starting point

Species-specific recognition provides evidence for a particular food-identity assay. It does not establish a validated disease test or performance for every mitochondrial sequence.

New research questions

Discuss selected mitochondrial sequences, variants and heteroplasmy research with a target-specific assay plan. These are research directions requiring new feasibility and validation work.

Build the controls

Assess sample preparation, specificity against nuclear mitochondrial DNA sequences (NUMTs), controlled mixture gradients and independent comparison methods.

Review mtDNA study scopeRead the published studyRead the correctionDiscuss collaboration
THE NEXT RESEARCH HORIZON

Molecular information.
Across time.

Our research thesis: reliable longitudinal molecular data is a critical missing layer for future AI-enabled health research.

TODAY · THE LABORATORY PLATFORM

Establish useful measurements

EFIRM96, EPLATE and software provide a laboratory platform for research and assay development. An automated prototype is available for demonstration and discussion with investment organizations and CLIA laboratories. It is a prototype, not a commercially available or clinically validated product.

FUTURE · SMALLER HARDWARE

Explore accessible sampling

Electrical control and current readout offer a route to miniaturization. Our longer-term goals include handheld instruments and smartphone modules, paired with appropriately validated assays.

FUTURE · LONGITUDINAL RESEARCH

Connect biology over time

Repeated saliva sampling could support molecular observations across time and physiological states. Carefully designed cohorts and reproducible measurements could create a data layer for future model-based research.

The path requires hardware development, assay validation, longitudinal studies and responsible data analysis. Handheld and smartphone products, continuous monitoring and AI-based clinical interpretation are future research goals.

Help develop the next research platform

05 / BUILD WHAT COMES NEXT

A shared pursuit
of better understanding.

01

For Customers

Explore EFIRM96, EPLATE and software for research and assay kit development in research institutions and Clinical Laboratory Improvement Amendments (CLIA) laboratories.

Explore research purchasing02

For Collaborators

Bring a scientific question. Connect on joint research, independent validation and translational studies, worldwide.

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For Investors

Review the platform foundation, research opportunities and diligence topics for professional funds and family offices.

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Research use only. Not for use in diagnostic procedures.
CLIA laboratory status does not itself establish authorization for an EFIRM assay.

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